Enhanced luminescence enzyme immunoassay for factor VIII related antigen.

نویسندگان

  • H X Wang
  • J George
  • G H Thorpe
  • R A Stott
  • L J Kricka
  • T P Whitehead
چکیده

A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).

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عنوان ژورنال:
  • Journal of clinical pathology

دوره 38 3  شماره 

صفحات  -

تاریخ انتشار 1985